Nature research paper: Intrinsic dopamine and acetylcholine dynamics in the striatum of mice
, merged image overlaid with DAPI nuclear stain . Similar results were obtained in= 16 other brains recovered for post-hoc validation of GRAB sensor expression and fiber optic placement., Epifluorescence images of immuno-enhanced rDA1m and ACh3.0 expressed in the dorsal striatum. Note the presence of rDA1m- and ACh3.
Note the localization of both sensors to the somatic membranes of striatal neurons and throughout the neuropil, and their exclusion from corticofugal axon bundles . Note also that some rDA1m forms intracellular aggregates., Same as c for a sagittal section of the substantia nigra pars reticulata. Subcellular localization of immuno-enhanced rDA1m and ACh3.0 by high magnification confocal microscopy was independently confirmed in four mice.
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