Nature research paper: Injury prevents Ras mutant cell expansion in mosaic skin
-stacks were acquired in 2–3 μm steps to image a total depth of 90–900 μm of the tissue. ImSpector v7.5.2 was used for 3D image acquisition. To visualize large areas, 5–12 tiles of optical fields were imaged using a motorized stage to automatically acquire sequential fields of view as previously described
. Mice were imaged at different time points after tamoxifen treatment and injury induction as indicated. To revisit the same area of the skin epidermis, organizational clusters of hair follicles and vasculature were used as landmarks.To prepare whole mounts of mouse ear, skin was separated from connective tissue using forceps and incubated in 4% paraformaldehyde at 37 °C for 4 h.
Argentina Últimas Noticias, Argentina Titulares
Similar News:También puedes leer noticias similares a ésta que hemos recopilado de otras fuentes de noticias.
A meta-analysis of GFR slope as a surrogate endpoint for kidney failure - Nature MedicineA meta-analysis of individual-level patient data from 66 clinical studies supports the utility of glomerular filtration rate as a surrogate endpoint in clinical trials for chronic kidney disease, with potential to enable detection of events earlier in the disease course.
Leer más »
Countries back contested EU nature law, seek more biodiversity fundingEuropean Union countries' environment ministers struck a deal on Tuesday over a landmark bill to restore deteriorating natural habitats after watering down parts of the proposal and agreeing to find more EU funding to restore damaged environments.
Leer más »
Sequential and directional insulation by conserved CTCF sites underlies the Hox timer in stembryos - Nature GeneticsTemporal activation of Hox genes in mouse pseudo-embryos in vitro initiates at the anterior part of the cluster and is accompanied by asymmetric loading of cohesin. Posterior CTCF sites then successively act as transient insulators, regulating the timed transcription of more posterior-located genes.
Leer más »
Local Opinion: AI and Mother Nature'AI is not holding its breath; neither is Mother Nature.'
Leer más »
Portugal to swap $153 mln Cape Verde debt for nature investmentsPortugal plans to swap the entire 140 million euro ($152.91 million) debt it is owed by Cape Verde for investments in the archipelago's environmental and climate fund, Prime Minister Antonio Costa said on Tuesday.
Leer más »
Combining 47 human genomes into a single pangenome - Nature BiotechnologyNature Biotechnology - Combining 47 human genomes into a single pangenome
Leer más »