Advancing Structural Biology With Novel Cell-Free Protein Crystallization Method

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Advancing Structural Biology With Novel Cell-Free Protein Crystallization Method
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Tokyo Tech developed a new cell-free protein crystallization (CFPC) method that includes direct protein crystallization and is a major advancement in the field of structural biology. This technique will enable the analysis of unstable proteins that couldn't be studied using conventional methods. Ana

Schematic illustration of CFPC process using a wheat germ protein synthesis kit to synthesis polyhedrin monomer which was further crystallized to nano-sized polyhedra crystals. Credit: Prof. Takafumi Uenoincludes direct protein crystallization and is a major advancement in the field of structural biology. This technique will enable the analysis of unstable proteins that couldn’t be studied using conventional methods.

Scanning electron microscopy images and size distribution histograms of polyhedra crystals show various points in the time-dependent CFPC process. Credit: Prof. Takafumi Ueno, the team reported the development of a technique that would make protein crystallization and analysis more efficient and effective.

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