Quantification of absolute transcription factor binding affinities in the native chromatin context using BANC-seq
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Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Heatmap showing copy numbers per cell or nucleus of detected transcription factors before and after nuclear isolation. Anti-FLAG western blot of FLAG-YY1 in nuclei or protein incubation buffer, immediately after nuclear permeabilization and after 10 minutes of incubation, with or without nuclear permeabilization by pulse sonication.
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