Nature research paper: Origin and evolutionary malleability of T cell receptor α diversity
) and adult trout were obtained from fish dealers. The blood samples from three female adult African bush elephants were obtained from the Wuppertal Zoological Garden and provided by L. Grund. All animal experiments were performed in accordance with relevant guidelines and regulations, approved by the review committee of the Max Planck Institute of Immunobiology and Epigenetics and the Regierungspräsidium Freiburg, Germany .Animals were euthanized using 0.02% MESAB.
Mouse lymphocytes were obtained from either the thymus or the spleen ; cells were passed through a cell strainer in PBS, centrifuged and the cell pellet dissolved in TRIzol following the recommendations of the manufacturer. For elephant blood samples, mononuclear cells were isolated from roughly 50 ml of peripheral blood as described in ref.Percoll condition; cells were washed and resuspended in TRIzol. Total RNAs were extracted from TRIzol according to the manufacturer’s protocol..
Argentina Últimas Noticias, Argentina Titulares
Similar News:También puedes leer noticias similares a ésta que hemos recopilado de otras fuentes de noticias.
The Origin Of Godzilla's Infamous Dance Scene ExplainedGodzilla has made some head-scratching moves in his history, but none is as baffling as his infamous dance scene. Here's where it came from.
Leer más »
DNA has revealed the origin of this giant ‘mystery’ geckoA genetic analysis of a 19th century museum specimen, the only known example of the planet’s biggest gecko, has rewritten the animal’s backstory.
Leer más »
Sony's Kraven Seriously Just Stole Another Supervillain's Origin From DC?!The Kraven The Hunter movie trailer raises the question of whether the Sony movie star is using a DC supervillain's backstory as his own.
Leer más »
Democratizing knowledge representation with BioCypher - Nature BiotechnologyNature Biotechnology - Democratizing knowledge representation with BioCypher
Leer más »
Activists, ranchers clash over proposed changes to Point Reyes nature preserveFor several years during the drought, environmentalists criticized the National Park service for its management of a Tule Elk reserve at Tomales Point in western Marin County. But recently, a change was announced to the management plan that has the entire region up in arms.
Leer más »