Nature research paper: Noncoding translation mitigation
, Growth defect of BAG6 KO cells and RNF126 KO cells revealed by competitive growth assays. KO cells and WT cells were mixed and co-cultured for 15 days and the relative cell numbers at each time point was determined by decomposition of sanger sequencing traces as described in Methods. N = 1 for day 0 of BAG6 and N = 3 biologically independent samples for all other time points. Data are presented as mean values +/− s.d.
Samples were run separately under denaturing conditions for immunoblot probing for actin as a sample processing control ., The level of 26S 1- and 2-cap proteasome detected by immunoblotting normalized to actin in the same sample , densitometric quantification of 26S 1- and 2-cap proteasome in-gel activity normalized by actin in the same sample , and the activity/level ratio .
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