The April issue is live On our cover, Volpato et al. have developed a method that extends fluorescence anisotropy measurements to large protein complexes
The addition of Cas9 target sequences to long single-stranded DNAs, combined with cocktails of small molecules to boost homology-directed repair, leads to marked enhancement of non-viral knock-in efficiency and yield in primary human T cells and other hematopoietic cell types.
The ability to target the majority of human transcription factors remains an as yet unachieved goal in chemical biology and medicine. We developed a modular, synthetic transcriptional repressor platform that recapitulates the DNA-binding properties of native basic helix-loop-helix domains and can block the DNA-binding functions of an important oncogenic transcription factor target, MYC.
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