Lignocellulose bio-refinery developed for value-added chemical overproduction in yeast

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Lignocellulose bio-refinery developed for value-added chemical overproduction in yeast
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Lignocellulosic biomass is a renewable feedstock for 2nd-generation biomanufacturing. In particular, efficient co-fermentation of mixed glucose and xylose in lignocellulosic hydrolysates is a key issue in reducing product costs.

and 3-hydroxypropionic acid , owing to the enhanced supply of precursor acetyl-CoA and cofactor NADPH by rewiring the cellular metabolism of Ogataea polymorpha.The researchers realized co-utilization of glucose and xylose by introducing a hexose transporter mutant and xylose isomerase, and overexpressing the native xylulokinase to enhance xylose catabolism and import.

The engineered strain produced 7.0 g/L FFA from real lignocellulosic hydrolysates in shake flasks and 38.2 g/L FFA from simulated lignocellulose in a bioreactor. Furthermore, this superior cell factory was expanded for 3-HP production by a metabolic transforming strategy, obtaining the highest 3-HP titer of 79.6 g/L from simulated lignocellulose.and glucose without compromising native glucose metabolism and demonstrated the potential of O.

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