Nature research paper: Intron-mediated induction of phenotypic heterogeneity
The 5′ UTR intron-deletion strain and its parental strain, with Rps22B–GFP fusion and constitutive mCherry expression, were grown overnight in YPD. For growth rate determination experiments in liquid media, 5 ml of each saturated overnight culture was standardized to ODLiCl in 12 replicates of each strain and condition. OD was measured every 5 min for 24 h in a plate reader during incubation at 30 °C with constant shaking. OD and growth rates were processed as above .
Plates were processed at 8, 12, 16, 20 and 24 h after inoculation with an automated robotic system consisting of a ACell System integrated with a Lynx liquid handling system , a plate reader and a CytoFLEX flow cytometer . First, plates were shaken and measured as described above to obtain the ODvalues. Cultures were then transferred to a new plate and, when necessary, diluted to an absorbance of around 0.3 with Tris-EDTA buffer to avoid high cell densities, and measured by flow cytometry.
Argentina Últimas Noticias, Argentina Titulares
Similar News:También puedes leer noticias similares a ésta que hemos recopilado de otras fuentes de noticias.
Agriculture and climate change are reshaping insect biodiversity worldwide - NatureInteraction between climate warming and intensive agricultural land use is associated with reductions in insect abundance and species richness, which can be mitigated by nearby natural habitats in low-intensity agricultural settings.
Leer más »
The Multi-Faceted Nature of SelfhoodResearchers think our inner identities are composed of several layers, though it isn't yet clear how exactly they operate. Find out more in one of our latest articles. Click the link below.
Leer más »
Opening of glutamate receptor channel to subconductance levels - NatureCryo-EM structures of AMPA receptor with the subunit γ2 in non-desensitizing conditions at low glutamate concentrations disprove the one-to-one link between the number of glutamate-bound subunits and ionotropic glutamate receptor conductance.
Leer más »
Somatic genomic changes in single Alzheimer’s disease neurons - NatureAnalyses of single-cell whole-genome sequencing data show that somatic mutations are increased in the brain of individuals with Alzheimer’s disease compared to neurotypical individuals, with a pattern of genomic damage distinct from that of normal ageing.
Leer más »
Global seasonal forecasts of marine heatwaves - NatureClimate forecast systems are used to develop and evaluate global predictions of marine heatwaves (MHWs), highlighting the feasibility of predicting MHWs and providing a foundation for operational MHW forecasts to support climate adaptation and resilience.
Leer más »
Increased Memory B Cell Potency and Breadth After a SARS-CoV-2 mRNA Boost - NatureNature - Increased Memory B Cell Potency and Breadth After a SARS-CoV-2 mRNA Boost
Leer más »