Discovery of a Ni2+-dependent guanidine hydrolase in bacteria - Nature

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Discovery of a Ni2+-dependent guanidine hydrolase in bacteria - Nature
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Nature research paper: Discovery of a Ni2+-dependent guanidine hydrolase in bacteria

were determined using different guanidine concentrations. Data points represent the average of n = 3 technical replicates and the black line represents the least-square fit to the Michaelis-Menten equation. Error bars represent s.d. The whole experiment was repeated with an independent enzyme preparation, which gave consistent results.Influence of Na-cacodylate on the activity of GdmH with 10 mM guanidine as substrate.

The entire analysis was repeated with independent enzyme preparations with consistent results. The inset shows a Coomassie-stained gel with the purified GdmH variants and the positions of molecular weight markers. For gel source data, see Supplementary Fig.Time-dependence of urea production by GdmH in the presence of 10 mM guanidine. Note that almost half of the substrate was hydrolyzed after 3 days of incubation.

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