New fluorescent sensor reveals a keyprotein involved in interactions between cells osaka_univ CommsBio
), HEK293T cells were individually transfected with expression plasmids and cultured for 24 h. The cells were then washed with PBS containing 1 mM EDTA and mixed in phenol red-free DMEM/F12 supplemented with 10% FBS. Cells were co-cultured in a 15 mL conical tube under slow rotation at room temperature for 8 h and seeded on a glass-bottomed dish coated with 0.1% PEI .
For FRET ratio imaging, Cerulean was excited with a 405-nm laser. Donor and acceptor emissions were collected at 460–500 nm and 515–615 nm, respectively. An intensity display mode image was created using MetaMorph software .incubator chamber fitted onto a TCS SP8 laser scanning confocal microscope . Images were acquired using the confocal microscope with a PL APO 40×/1.10 NA water-immersion or a PL APO CS2 63×/1.40 NA oil-immersion objective lens .
Argentina Últimas Noticias, Argentina Titulares
Similar News:También puedes leer noticias similares a ésta que hemos recopilado de otras fuentes de noticias.
Evidence for a consistent use of external cues by marine fish larvae for orientation - Communications BiologyIn the majority of tested marine teleost fish species, larvae rely on external cues for orientation. This finding is important to understand larval behaviour and its implementation in biophysical dispersal models used for sustainable management and conservation of marine resources.
Leer más »
Minnesota science teacher claims cell biology lessons are part of 'capitalist indoctrination'Mandi Jung, a 7th grade science teacher in Highland Park Middle School in Minnesota, claimed that cell biology lessons were part of 'capitalist indoctrination.'
Leer más »
DOJ Special Counsel Jack Smith Subpoenas Brad Raffensperger for Communications With TrumpNewly appointed DOJ special counsel Jack Smith has sent a grand jury subpoena to Georgia Secretary of State Brad Raffensperger, who had an infamous conversation where Trump ordered him to “find” him enough votes to overturn Biden’s win in Georgia.
Leer más »
A distinct ripple-formation regime on Mars revealed by the morphometrics of barchan dunes - Nature CommunicationsDust storms on Mars drive water escape to space. Here, the authors show the impact Martian dust storms have on the abundance of atmospheric hydrogen and oxygen, and how this helps to overall oxidize the Martian atmosphere.
Leer más »
Molecular basis for DNA recognition by the maternal pioneer transcription factor FoxH1 - Nature CommunicationsFoxH1 is an essential maternal pioneer factor during embryonic development. Here, the authors present several FoxH1—DNA structures that reveal the molecular basis for FoxH1 binding specificity and pioneer factor activity.
Leer más »
Raina Falcon Promoted To Oversee Communications For HBORaina Falcon is adding HBO to her slate. Previously, overseeing comms for HBO Max, Falcon becomes SVP, Communications, HBO & HBO Max Content. Reporting to Jeff Cusson, Head of Communications, S…
Leer más »