Nature research paper: Alternative CDC20 translational isoforms tune mitotic arrest duration
Sensitivity of HeLa or M1-stop cells to increasing concentrations of Taxol, Nocodazole, or the CENP-E inhibitor GSK923295. Cell viability was determined by MTT assay in triplicate following 72 h drug treatment. Data are mean ± s.e.m from three or four experimental replicates. Statistics from Student’s two-sample t-Test with two-tailed distribution comparing HeLa and M1-stop cell viabilities per drug concentration .
Similar sensitivity assay as in except for STLC for representative clones of M1-stop mutant with low, medium, or high expression of the doxycycline-inducible wild-typeconstruct without induction or induced with 20 ng/ml doxycycline. Data are mean ± s.e.m from three experimental replicates. Statistics from Student’s two-sample t-Test with two-tailed distribution comparing the uninduced and induced cell viabilities per drug concentration .
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